Maintaining DNA integrity is critical for molecular analysis in various research areas. Scientists have conducted extensive studies to identify the most effective taxon-specific preservatives for DNA conservation. While highly concentrated ethanol has generally been shown to be effective, there are exceptions (Fukatsu 1999; Vink et al. 2005). In this blog post, we will look at the technical aspects of DNA preservation and explain how Clitec GmbH’s Climate Ultra Cold test chamber helps preserve DNA integrity, taking into account current research findings.

Challenges of taxon specific conservation

Determining the ideal preservation method for DNA collection from organisms in the wild presents challenges. Numerous studies have focused on identifying the best taxon specific preservative, taking into account factors such as applicability, regulatory restrictions, and evaporation rates (Waller and Strang 1996; King and Porter 2004; Gurdebeke and Maelfait 2002). Researchers have found that 95 % ethanol consistently yields the highest DNA concentration in ant samples and their associated microbes stored at room temperature (Fig. 1) (C. S. Moreau et al.). However, travel restrictions on ethanol at concentrations above 70 % in the United States must be considered, making propylene glycol an attractive alternative due to its chemical stability and lower evaporation rate (International Air Transport Association 2012; C. S. Moreau et al.).

The role of the Climate Ultra Cold test chamber from Clitec GmbH

The Climate Ultra Cold test chamber from Clitec GmbH addresses the challenges of taxon specific DNA preservation. By generating and maintaining ultracold temperatures as low as -80 °C, the test chamber provides an optimal environment for DNA storage. This controlled and stable environment prevents enzymatic activity and degradation and ensures long-term maintenance of DNA integrity.

Versatile preservation options

The Clitec GmbH test chamber offers researchers the flexibility to select their preferred preservative. While 95 % ethanol is the ideal preservative for ants and their associated microbes, propylene glycol is a chemically stable alternative (C. S. Moreau et al.). The test chamber allows the use of different preservatives so that researchers can select the most suitable option according to their specific requirements.

Effective workflow and sample management

The Climate Ultra Cold test chamber is designed to streamline workflow and facilitate sample management. With optimized interior partitioning, researchers can efficiently organize and retrieve DNA samples. The advanced monitoring and control systems of the test chamber ensure constant maintenance of ultracold temperatures and reduce the risk of temperature fluctuations that could affect DNA stability.

Compliance with quality assurance and regulations

Clitec GmbH places great emphasis on quality assurance and regulatory compliance in the design and manufacture of the Climate Ultra Cold test chamber. The chamber undergoes rigorous testing and meets industry-specific guidelines for laboratory equipment. Researchers can rely on the performance and compliance of the chamber and be assured that their DNA samples are preserved in a controlled and standardized environment.

Conclusion

Maintaining DNA integrity is critical for molecular analysis in various scientific disciplines. The Climate Ultra Cold test chamber from Clitec GmbH offers researchers an advanced solution for taxon-specific DNA preservation. By taking advantage of the test chamber’s temperature-controlled environment and versatile preservation options, researchers can effectively preserve DNA integrity and pave the way for breakthrough discoveries in genetic and genomic research. Recent studies have highlighted the efficacy of taxon specific preservatives such as 95 % ethanol and propylene glycol and confirmed the relevance and applicability of the Clitec GmbH test chamber in preserving DNA samples in various arthropods and their associated microbes.

Reference

Moreau, Corrie & Wray, Brian & Czekanski-Moir, Jesse & Rubin, Benjamin. (2013). DNA preservation: A test of commonly used preservatives for insects. Invertebrate Systematics. 27. 81. 10.1071/IS12067.